Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4)

Application

Frozen Sections (see application references)Immunoblotting (1 g/ml)Paraffin Sections (see comments and application references)

General description

Recognizes the ~57/59 kDa latent and the ~28/45 kDa active forms of MMP-3. Will also recognize MMP-3/TIMP-1 complexes. Slightly cross-reacts (~34%) with human MMP-10.

This Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-3 (Ab-1).

Purified mouse monoclonal antibody (see application references). Recognizes the ~59/57 kDa latent and the ~45/28 kDa active forms of MMP-3 and MMP-3/TIMP complexes (see application references).

Immunogen

human pro-MMP-3 from the conditioned media of rheumatoid synovial fibroblasts

Human

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.Woessner, J.F. 1991. FASEB J.5, 2145.Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.

This antibody has 34% cross-reactivity with human MMP-10. For paraffin sections, staining can be enhanced by culturing tissue in 2 µM monensin for 3 h. Pre-treating with trypsin or a pressure cooker may enhance staining. Tissue can then be fixed with PLP (perioddate-lysine-4% paraformaldehyde) and stained using 5-10 µg/ml MMP-3 antibody. See application references for more information. Clone 55-2A4 is called Clone B in Obata, K., et al. To prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Store in aliquots at -20°C. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic Bag(s)

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)